Post-Grant Reports

Title

Student-Faculty Collaborative Research Grant Report

Document Type

Report

Publication Date

2-15-2016

Disciplines

Analytical Chemistry | Biochemistry | Biology | Systems Biology

Abstract

Mitochondria are key organelles within cells because they house oxidative phosphorylation and are essential in the generation of the high-energy molecule ATP. They are also unique in that they also have their own small genome and their own set of transcription machinery to transcribe the mitochondrial DNA into RNA. The Faculty Student Collaborative Research fund provided support for a single student during the summer of 2015 for a project focused on developing a novel analytical method in the separation and quantification of DNA and RNA mixtures. Specifically, ion paired reverse phase (IP-RP) high performance liquid chromatography (HPLC) was used to separate and quantify DNA and RNA molecules based on size using different analysis parameters. As part of this project we have developed a protocol that is being applied to in vitro RNA transcription assays for quantitative analysis of RNA output under a given set of conditions. This is important for the progression of this research project in assessing RNA transcripts generated from linear mitochondrial DNA templates using specific proteins of interest.

Comments

This research was conducted as part of a Linfield College Student-Faculty Collaborative Research Grant in 2015, funded by the Office of Academic Affairs.

The student collaborator was Henry Wienkers.

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