Faculty Sponsor(s)
Brian Gilbert
Location
Jereld R. Nicholson Library: Grand Avenue
Subject Area
Chemistry
Description
Gold-core nanoparticles coated in silver were introduced to yeast cells as a part of an ongoing project to utilize functionalized nanoparticles as drug delivery vehicles. Nanoparticles were synthesized through a reduction method using trisodium citrate and characterized optically with a UV-vis spectrophotometer and NanoSight LM10HS particle sizer. The nanoparticles were coated in silver through a reduction method using AgNO3. Yeast cells were cultivated in a liquid YPD, yeast extract peptone dextrose, a medium used for yeast growth. The cells were centrifuged and washed for purification and characterized by calculating optical density with the spectrophotometer. For maximum intake, nanoparticles were incubated with cells after 24 hours of yeast cell growth, before reaching a stationary growth phase.
Recommended Citation
Alfaro, Ana; Gilbert, Brian; and Falcon Ontiveros, Fatima, "Lipid-Coated Core-Shell Noble Metal Nanoparticles" (2018). Linfield University Student Symposium: A Celebration of Scholarship and Creative Achievement. Event. Submission 6.
https://digitalcommons.linfield.edu/symposium/2018/all/6
Lipid-Coated Core-Shell Noble Metal Nanoparticles
Jereld R. Nicholson Library: Grand Avenue
Gold-core nanoparticles coated in silver were introduced to yeast cells as a part of an ongoing project to utilize functionalized nanoparticles as drug delivery vehicles. Nanoparticles were synthesized through a reduction method using trisodium citrate and characterized optically with a UV-vis spectrophotometer and NanoSight LM10HS particle sizer. The nanoparticles were coated in silver through a reduction method using AgNO3. Yeast cells were cultivated in a liquid YPD, yeast extract peptone dextrose, a medium used for yeast growth. The cells were centrifuged and washed for purification and characterized by calculating optical density with the spectrophotometer. For maximum intake, nanoparticles were incubated with cells after 24 hours of yeast cell growth, before reaching a stationary growth phase.
