Faculty Sponsor(s)
Elizabeth Atkinson
Location
Jereld R. Nicholson Library: Grand Avenue
Subject Area
Chemistry
Description
Silica sol-gels synthesized through hydrolysis and condensation reactions via acid- and base-catalyzed procedures containing 4’,6-diamidino-2-phenylindole (DAPI) have been examined using fluorescence spectroscopy. DAPI is a fluorescent molecule that has traditionally been used in biosensors as a target molecule and a fluorescent stain known to bind strongly to the A-T rich regions of DNA. Sol-gels containing various concentrations of DAPI were dried conventionally to form xerogels or supercritically to form aerogels and then analyzed using fluorescence spectroscopy to determine the most optimal concentration of DAPI.
Recommended Citation
Trujillo, Nalea and Atkinson, Elizabeth, "Examination of 4’,6-diamidino-2-phenylindole (DAPI) in Silica Gels through Fluorometry" (2016). Linfield University Student Symposium: A Celebration of Scholarship and Creative Achievement. Event. Submission 1.
https://digitalcommons.linfield.edu/symposium/2016/all/1
Examination of 4’,6-diamidino-2-phenylindole (DAPI) in Silica Gels through Fluorometry
Jereld R. Nicholson Library: Grand Avenue
Silica sol-gels synthesized through hydrolysis and condensation reactions via acid- and base-catalyzed procedures containing 4’,6-diamidino-2-phenylindole (DAPI) have been examined using fluorescence spectroscopy. DAPI is a fluorescent molecule that has traditionally been used in biosensors as a target molecule and a fluorescent stain known to bind strongly to the A-T rich regions of DNA. Sol-gels containing various concentrations of DAPI were dried conventionally to form xerogels or supercritically to form aerogels and then analyzed using fluorescence spectroscopy to determine the most optimal concentration of DAPI.
