Progress Toward Synthesis of Derivatives of Arp2/3 Complex Inhibitor CK-666
Faculty Sponsor(s)
Andrew Baggett
Subject Area
Chemistry
Description
The Actin related Protein (Arp 2/3) is responsible for nucleating new actin filaments to create a branched network of actin. This activity is crucial for processes such as cell movement, endocytosis, and intracellular trafficking. The objective of the investigation is to establishing a reversible method of inhibiting the Arp 2/3 complex based on the known small-molecule Arp2/3 inhibitor CK-666. The use of CK-666 can prevent the motility of harmful pathogens across the cytoplasm of model organism cells. This project is also designed to provide a tool of use to researchers investigating the basic science of actin. The inhibitory strength of synthesized derivatives of CK-666 will be determined through a fluorescence assay to generate an IC50 value. The goal of the investigation involves developing an inhibitor with a lowered IC50 value. This research describes organic synthesis and biochemical testing of a derivative of CK-666 and synthetic efforts towards an azaindole derivative of CK-666.
Recommended Citation
Fernandez, Allan and Baggett, Andrew, "Progress Toward Synthesis of Derivatives of Arp2/3 Complex Inhibitor CK-666" (2025). Linfield University Student Symposium: A Celebration of Scholarship and Creative Achievement. Event. Submission 1.
https://digitalcommons.linfield.edu/symposium/2025/all/1
Progress Toward Synthesis of Derivatives of Arp2/3 Complex Inhibitor CK-666
The Actin related Protein (Arp 2/3) is responsible for nucleating new actin filaments to create a branched network of actin. This activity is crucial for processes such as cell movement, endocytosis, and intracellular trafficking. The objective of the investigation is to establishing a reversible method of inhibiting the Arp 2/3 complex based on the known small-molecule Arp2/3 inhibitor CK-666. The use of CK-666 can prevent the motility of harmful pathogens across the cytoplasm of model organism cells. This project is also designed to provide a tool of use to researchers investigating the basic science of actin. The inhibitory strength of synthesized derivatives of CK-666 will be determined through a fluorescence assay to generate an IC50 value. The goal of the investigation involves developing an inhibitor with a lowered IC50 value. This research describes organic synthesis and biochemical testing of a derivative of CK-666 and synthetic efforts towards an azaindole derivative of CK-666.
Comments
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