Elucidating the role of Exo1 and Mre11 mutations in DNA damage response
Faculty Sponsor(s)
Sreerupa Ray
Location
Jereld R. Nicholson Library: Grand Avenue
Subject Area
Biology
Description
DNA double strand breaks (DSBs) are one of the most deleterious types of DNA damage that cells combat. DSBs are processed by the 3′-to-5′ exonuclease activity of the DSB repair nuclease, Mre11, to generate protruding 3′ single stranded DNA (ssDNA) at DSBs. Exonuclease 1 (EXO1) is an evolutionarily, well-conserved exonuclease. Its ability to resect DNA in the 5′-3′ direction has been extensively characterized and shown to be implicated in several genomic DNA metabolic processes such as replication stress response, double strand break repair (DSBR), mismatch repair, nucleotide excision repair and telomere maintenance. Both Mre11 and Exo1 play a critical role in the DNA resection in DSBR. Mre11 is responsible for the initial short-range resection, which is followed by the long resection by Exo1 before strand invasion in DSBR. However, the regulation of this switch between Mre11 and Exo1 is not well understood. The purpose of this study is to test the hypothesis that EXO1 and MRE11 gene variants contribute to genomic instability by aberrant DNA repair. This project seeks to understand the potential role of disease-associated variants of Exo1 and Mre11 in defective resection function, thus inducing genomic instability.
Recommended Citation
Remmick, Frances; Morales, Andrea Quintana; Davis, Kylie; Miyama, Ariel; and Ray, Sreerupa, "Elucidating the role of Exo1 and Mre11 mutations in DNA damage response" (2022). Linfield University Student Symposium: A Celebration of Scholarship and Creative Achievement. Event. Submission 19.
https://digitalcommons.linfield.edu/symposium/2022/all/19
Elucidating the role of Exo1 and Mre11 mutations in DNA damage response
Jereld R. Nicholson Library: Grand Avenue
DNA double strand breaks (DSBs) are one of the most deleterious types of DNA damage that cells combat. DSBs are processed by the 3′-to-5′ exonuclease activity of the DSB repair nuclease, Mre11, to generate protruding 3′ single stranded DNA (ssDNA) at DSBs. Exonuclease 1 (EXO1) is an evolutionarily, well-conserved exonuclease. Its ability to resect DNA in the 5′-3′ direction has been extensively characterized and shown to be implicated in several genomic DNA metabolic processes such as replication stress response, double strand break repair (DSBR), mismatch repair, nucleotide excision repair and telomere maintenance. Both Mre11 and Exo1 play a critical role in the DNA resection in DSBR. Mre11 is responsible for the initial short-range resection, which is followed by the long resection by Exo1 before strand invasion in DSBR. However, the regulation of this switch between Mre11 and Exo1 is not well understood. The purpose of this study is to test the hypothesis that EXO1 and MRE11 gene variants contribute to genomic instability by aberrant DNA repair. This project seeks to understand the potential role of disease-associated variants of Exo1 and Mre11 in defective resection function, thus inducing genomic instability.
