Faculty Sponsor(s)
Megan Bestwick
Location
Jereld R. Nicholson Library: Grand Avenue
Subject Area
Chemistry
Description
TOR1 is known to extend chronological lifespan in S. cerevisiae. Δtor1 deletion was studied via chronological lifespan assay and environmental oxidative stress test. This deletion was confirmed via growth in selective media and through deletion check primers. The Δtor1 deletion created demonstrated similar growth and cellular response to environmental oxidative stress as previously put forth by Bonawitz et al. and Pan et al.
In this work, partial inhibition of TOR by deletion of TOR1 is studied through S. cerevisiae longevity via a chronological lifespan assay, with intention of studying the double gene deletion strains of Δlys7/Δtor1, Δsod1/Δtor1, Δsod2/Δtor1, and Δctr1/Δtor1. Double gene deletion strains of Δlys7/Δtor1, Δsod1/Δtor1, Δsod2/Δtor1, and Δctr1/Δtor1 are not well-characterized for their effects on S. cerevisiae longevity.
Recommended Citation
Keyes, Amelia and Bestwick, Megan, "Effects of Δtor1 on Yeast Longevity via Chronological Lifespan Assay" (2017). Linfield University Student Symposium: A Celebration of Scholarship and Creative Achievement. Event. Submission 36.
https://digitalcommons.linfield.edu/symposium/2017/all/36
Effects of Δtor1 on Yeast Longevity via Chronological Lifespan Assay
Jereld R. Nicholson Library: Grand Avenue
TOR1 is known to extend chronological lifespan in S. cerevisiae. Δtor1 deletion was studied via chronological lifespan assay and environmental oxidative stress test. This deletion was confirmed via growth in selective media and through deletion check primers. The Δtor1 deletion created demonstrated similar growth and cellular response to environmental oxidative stress as previously put forth by Bonawitz et al. and Pan et al.
In this work, partial inhibition of TOR by deletion of TOR1 is studied through S. cerevisiae longevity via a chronological lifespan assay, with intention of studying the double gene deletion strains of Δlys7/Δtor1, Δsod1/Δtor1, Δsod2/Δtor1, and Δctr1/Δtor1. Double gene deletion strains of Δlys7/Δtor1, Δsod1/Δtor1, Δsod2/Δtor1, and Δctr1/Δtor1 are not well-characterized for their effects on S. cerevisiae longevity.
