Student-Faculty Collaborative Research Grant Report
Analytical Chemistry | Biochemistry | Biology | Systems Biology
Mitochondria are key organelles within cells because they house oxidative phosphorylation and are essential in the generation of the high-energy molecule ATP. They are also unique in that they also have their own small genome and their own set of transcription machinery to transcribe the mitochondrial DNA into RNA. The Faculty Student Collaborative Research fund provided support for a single student during the summer of 2015 for a project focused on developing a novel analytical method in the separation and quantification of DNA and RNA mixtures. Specifically, ion paired reverse phase (IP-RP) high performance liquid chromatography (HPLC) was used to separate and quantify DNA and RNA molecules based on size using different analysis parameters. As part of this project we have developed a protocol that is being applied to in vitro RNA transcription assays for quantitative analysis of RNA output under a given set of conditions. This is important for the progression of this research project in assessing RNA transcripts generated from linear mitochondrial DNA templates using specific proteins of interest.
Bestwick, Megan, "Student-Faculty Collaborative Research Grant Report" (2016). Post-Grant Reports. Report. Submission 68.